The part of the project coordinated by our research group ate University Jaume I, SMART-2PM, aims to perform high-resolution imaging of biological samples with wide-field 2-photon microscopy (also known as “non-linear microscopy”) based on light structured illumination and integrated detection. The system will combine new fs-light sources in the mid-IR, sophisticated adaptive optics systems, temporal focusing, single-pixel imaging strategies, and computational imaging approaches based on machine learning for adaptive detection. We will fix the fundamental trade-off between penetration depth, sensitivity, and imaging speed of current nonlinear microscopes for imaging biological tissues by applying, simultaneously, adaptive optics and computational imaging strategies.